The mycoalert tm mycoplasma detection kit 100 tests is a selective biochemical test that exploits the activity of mycoplasmal enzymes which are found in all six of the main mycoplasma cell culture contaminants and the vast majority of 180 mycoplasma. All components required for the pcr reaction are provided. The kit incorporates polymerase chain reaction pcr to amplify the conserved 16s ribosomal rna coding region within the mycoplasma genome, thereby. The kit contains a readytouse pcr reaction mix with highlyoptimized mycoplasma specific primers, a positive control, and an internal. The universal mycoplasma detection kit offers a quick and sensitive pcrbased test to detect myco plasma contaminants in cell culture. The test is specific to a broad range of mycoplasma.
Mycoseq mycoplasma realtime pcr detection kit user guide. Strict adherence to good laboratory practices such as good aseptic technique are key, and routine testing for mycoplasma is highly recommended for successful control of mycoplasma contamination. Pdf comparison of mycoplasma ies, mycofast revolution. Kit has been successfully tested for the ability to amplify mycoplasma. The pcr mycoplasma test kit is designed to detect the presence of mycoplasma contaminating biological materials, such as cultured cells. Buy ezpcr detection kit, get aquaguad 1 or 2 free offer valid in israel only. Pcr method allows for fast and reliable identification of mycoplasma contamination in cell cultures. The primer set amplifies a dna sequence within the highly conserved.
These eight species account for approximately 95% of all mycoplasma. Mycoplasma pneumonia igm elisa kit background information. Mycoplasma detection by the direct culture procedure is timeconsuming and some mycoplasma. This autobio kit is used for culture isolation, identification, enumeration of ureaplasma urealyticum uu and mycoplasma hominis mh and clinical susceptibility of 12 commonly used antibiotics. The primers have been designed to detect those mycoplasma species responsible for most contaminations in cell cultures including acholeplasma and spiroplasma and were tested to be specific for mycoplasma dna only, not reacting with animal or bacterial dna. The lonza mycoalert mycoplasma detection kit is a simple mycoplasma detection kit. Infections usually result in mycoplasma titers of 105 108 cfuml the kit allows detection of acholeplasma and spiroplasma and the following mycoplasma. Ureaplasma spp ureaplasma urealyticum, ureaplasma parvum and mycoplasma hominismh.
The detection by pcr requires only 1 to 5 femtograms of mycoplasma dna corresponding to only 25 mycoplasma. Find and purchase mycoplasma detection kits products at invitrogen life science technologies. The lookout mycoplasma pcr detection kit utilizes the polymerase chain reaction pcr, which is established as the method of choice for highest sensitivity in the detection of mycoplasma and acholeplasma contamination in cell cultures and other cell culture derived biologicals. The universal mycoplasma detection kit offers a quick and sensitive pcrbased test to detect mycoplasma contaminants in cell culture. Pcr mycoplasma test kit readytouse pcr mix for the detection of mycoplasma in cell culture productno. For the lonza mycoplasma kit, which wavelength should i use to read my plate in the luminometer. Mycoplasma ist 3 simple and complete diagnosis of urogenital mycoplasmas. The kit includes a readytouse pcr reaction mix containing taq dna polymerase, dntps, and a highlyoptimized primer set, pcr buffer solution, and a positive. Bands of various lengths that are less intensive can be produced, but do not. Mycoplasma are common and serious contaminants of cell cultures. With pcr testing, results are obtained within a few hours, since the presence of contaminant mycoplasma.
Immunocard mycoplasma is a rapid, sensitive enzyme immunoassay for the detection of igm to mycoplasma pneumoniae in human serum. The pcr mycoplasma test kit is designed to detect the presence of mycoplasma contamination in biological materials, such as cultured cells. When performing pcr mycoplasma testing there are theoretically 40 strains that can be detected from a test article or sample. The kit contains a readytouse pcr reaction mix with highlyoptimized mycoplasma. The assay using this product can detect mycoplasma within a few hours by pcr and electrophoresis, unlike the cell culture assay, which requires about one week. Mycoplasma detection kit instruction ezpcr mycoplasma detection kit is designed to detect the presence of mycoplasma, since it is common contaminant of biological materials including cultured cells. Mycoplasma detection by the direct culture procedure is timeconsuming and some mycoplasma species are difficult to cultivate. The mycosensor qpcr assay kit includes a primer mix that amplifies the eight most common species of mycoplasma. Mycoseq mycoplasma realtime pcr detection kits user guide. Mycoplasma pneumoniae is among the smallest of freeliving organisms. Ezpcr mycoplasma detection kit biological industries. Detecting mycoplasma using the mycoalert kit on the. This simple organism can drastically affect cell viability and growth, along with multiple other characteristics, and can be very difficult to detect.
Mycoplasma ist 3 combines enumeration, identification and antibiotic susceptibility testing for detection of mycoplasma. Ezpcr mycoplasma test kit is designed to detect the presence of mycoplasma contaminating biological materials, such as cultured cells. The pcr mycoplasma test kit is adequate to diagnose cell cultures infected with mycoplasmas. Validation of the mycotool mycoplasma realtime pcr kit. Mycoplasma ist 3, simple and complete diagnosis of. Product overview the mycoalert tm plus assay is a selective biochemical test that exploits the activity of mycoplasmal enzymes which are found in all six of the main mycoplasma cell culture contaminants and the vast majority of 180 mycoplasma species, but are not present in eukaryotic cells. Mycoplasma contamination can cause the alteration of the phenotypic cell characteristics. Pcr mycoplasma test kit ii is designed to detect the presence of mycoplasma contamination in biological materials, such as cultured cells. The mycosensor pcr and qpcr assay kits detect mycoplasma infection of cell cultures by realtime quantitative pcr qpcr or pcr in less than two hours. Mycoplasma detection pcr method ezpcr mycoplasma test. Not for us e in diagnostic procedures for clinical purposes. The mycoplasma test kit irt for quantitative pcr is a high sensitive and fast test system for detection of mycoplasma contamination in cell cultures and other cell culture derived biologicals.
Mycoalert plus mycoplasma detection kit 100 tests lonza. Abstract the united states pharmacopeia has recently published chapter mycoplasma tests. Mycoplasma detection kits thermo fisher scientific. With immunocard your core lab, hospital lab, or reference. Covid19 iggigm rapid test whole blood serumplasma is a rapid chromatographic immunoassay for the qualitative detection of igg and igm antibodies against sarscov2 in human whole blood, serum or plasma. Bands of various lengths that are less intensive can be produced, but do not indicate positive results. The test reaction tubes contain all master mix reagents required for conventional polymerase chain reactions. Possible primer selfannealing produces another band of 8090 bp in length, but also does not. If you have additional questions about mycoplasma testing, or would like to. The easytouse system can specifically detect the eight most common mycoplasma. The ezpcr mycoplasma detection kit is a highlysensitive and specific pcr assay designed to test for the presence of over 90 species of mycoplasma, acholeplasma, and spiroplasma in cell cultures with a detection limit of 10 cfuml. What is the best way to check for mycoplasma in cell culture. Lookout mycoplasma pcr detection kit mp0035 technical.
There are two standard types of testing for mycoplasma contamination. The mycotool mycoplasma realtime pcr kit mycotool rt is a. The pcr mycoplasma test kit has been designed for high sensitivity and is therefore prone to nonspecific annealing. In humans, it is usually found in the throat and lungs and is. A3744 introduction the pcr mycoplasma test kit is designed to detect the presence of mycoplasma.
Pcr kits for sensitive and rapid detection of mycoplasmas in cell culture. The mycoprobe mycoplasma detection kit detects the most common mycoplasma cell culture contaminants, including m. The mycoalert kit is intended f or the quick and convenient detection of viable mycoplasma in cell cultures. This kit contains an optimized, complete pcr mix, including a variety of mycoplasma. Portable document format pdf versions of this guide and the documents listed above. Mycoplasma detection in cell cultures has until now been a long, drawn out process with difficulttointerpret results. Regulatory aspects of mycoplasma testing for cell therapy 2 2014 labs, inc. Ezpcr mycoplasma kit for the detection of mycoplasma in. Regulatory aspects of mycoplasma testing for cell therapy.
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